A Class book of Elementary, Practical Physiology; Including Histology, Chemical And Experimental Physiology

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Hssmalum. Hsematein can be purchased, and a moderately deep-coloured solution is made in a 10 '"=• solution of alum ; or dissolve hsematoxylin crystals in strong ammonia, dry in air, and make a, solution as above. This is one of the best nuclear bulk stains.
Requires four or more days, according to the density and size of the tissue treated. It does not overstain. Followed by increasing strengths of alcohol to the last of them, a small quantity of eosine can be added to give a ground stain befo
...re passing the tissue into cedar oil for paraffin embedding.
23. 'Weig'ept-Pal method, Bolton's modification (Jl. Anat. and Physiol., vol. xxxii., p. 264). For brain and spinal cord.
1. Fixing and hardening. The tissue as fresh as possible is placed in a large quantity of 5 f-"- formaldehyde (1 formol to 7 parts of water). Change occasionally for six weeks ; may remain for six months or longer. The tissue may be cut at the end of a week, but the sections have a slight tendency to frill.
2. Cutting.


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