A Compend On Bacteriology: Including Animal Parasites
A Compend On Bacteriology: Including Animal Parasites
Robert Lucas Pitfield
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57. ) Lockjaw Bacillus. First seen by Nicolaier, and isolated in pure culture by Kitasato. FIG. 57. Tetanus bacilli showing end spores. (Kolle and Wassermann. ) Morphology and Stains. Rod-shaped. Varying from 1. 2 p in length, to very long threads of 20 to 40/1. Sometimes grow in chains; frequently appear like short drum-sticks with a spore at one TETANUS BACILLUS 169 end, which is either round or oval. At times, the bacilli in chains sporulate. The organism is motile; possesses numerous flagel...la (from fifty to a hundred) peritrichously arranged; stains well with all the common basic aniline dyes, and retains the color in Gram's method. (Fig. 58. ) Oxygen Requirements. Strictly anaerobic when freshly iso- lated from earth or wounds, but, after long cultivation on culture media, it becomes more tolerant to small amounts of oxygen. Temperature. Grows best at 37 C. Below 14 C. Not at all. Vital Resistance. Spores resist 80 C. For an hour. This fact enabled Kitasato to kill all other organisms, except their spores, in pus.
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