A Course of Elementary Practical Bacteriology Including Bacteriological Analysi
A Course of Elementary Practical Bacteriology Including Bacteriological Analysi
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To quickly assess the difficulty of the text, read a short excerpt:
(b) Wash it rapidly in water and decolourise it in 25 per cent hydrochloric acid, and then proceed as above.
This method gives extremely good results, (iii. ) Gram's Method.
(a) Stain a few sections first with picrocarmine in the ordinary manner (vide p. 36).
(b) Place them in aniline gentian-violet for twenty-four hours.
(c) Wash a section rapidly in water.
64 GENERAL BACTERIOLOGY LESSON (d) Place it in Gram's iodine solution for one to two minutes.
(e) Wash it in absolute alcohol for half a m...inute and then in acid alcohol (3 per cent hydrochloric acid) for not longer than eight to ten seconds.
(/) Again wash it in absolute alcohol till no more stain comes off, and the specimen appears red.
(g) Dehydrate it in absolute alcohol.
(h) Clear it in xylol and mount it in xylol balsam.
Examine it with a high power and -^ in. Oil im- mersion. The bacilli are stained blue on a red ground.
Staining of Paraffin Sections (1) Tubercle. (i. ) Slow Method.
(a) Fix a section on a clean cover-glass (if possible with- out egg albumen or any other fixing medium) and remove the paraffin in the usual manner (heat, xylol, alcohol, and water).
What to read after A Course of Elementary Practical Bacteriology Including Bacteriological Analysi?
You can find similar books in the "Read Also" column, or choose other free books by Alfredo Antunes Kanthack to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
(b) Wash it rapidly in water and decolourise it in 25 per cent hydrochloric acid, and then proceed as above.
This method gives extremely good results, (iii. ) Gram's Method.
(a) Stain a few sections first with picrocarmine in the ordinary manner (vide p. 36).
(b) Place them in aniline gentian-violet for twenty-four hours.
(c) Wash a section rapidly in water.
64 GENERAL BACTERIOLOGY LESSON (d) Place it in Gram's iodine solution for one to two minutes.
(e) Wash it in absolute alcohol for half a m...inute and then in acid alcohol (3 per cent hydrochloric acid) for not longer than eight to ten seconds.
(/) Again wash it in absolute alcohol till no more stain comes off, and the specimen appears red.
(g) Dehydrate it in absolute alcohol.
(h) Clear it in xylol and mount it in xylol balsam.
Examine it with a high power and -^ in. Oil im- mersion. The bacilli are stained blue on a red ground.
Staining of Paraffin Sections (1) Tubercle. (i. ) Slow Method.
(a) Fix a section on a clean cover-glass (if possible with- out egg albumen or any other fixing medium) and remove the paraffin in the usual manner (heat, xylol, alcohol, and water).
What to read after A Course of Elementary Practical Bacteriology Including Bacteriological Analysi?
You can find similar books in the "Read Also" column, or choose other free books by Alfredo Antunes Kanthack to read onlineMoreLess
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