A Course of Elementary Practical Bacteriology Including Bacteriological Analysis
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(c) Prepare also cover-glass specimens of the spleen and peritoneal fluid, and stain them with Loffler's methylene- blue, and examine them with - in. Oil immersion.
Quick Method of Hardening and of Embedding in Paraffin (1) Place small pieces of the mouse's spleen, kidney, liver, and lung in absolute alcohol.
(2) Change the alcohol every half -hour, using always an excess.
(3) After one and a half to two hours place the small pieces of tissue in a small corked bottle containing benzol, till the...y are transparent (a few minutes).
(4) Now pour off the excess of benzol, so that the pieces are only just covered by it, add a few shavings of paraffin (56 to 60 C. Melting point), and place the bottle on the paraffin stove, till the paraffin liquefies.
134 BACTERIOLOGICAL ANALYSIS LESSON (5) Transfer the specimens to liquid hard paraffin (56 to 60 C. ), and allow them to soak for five minutes.
(6) Pour the paraffin and the tissues into a small paper box, and when the paraffin is set, cut out the pieces of tissue, fix them on the rocking microtome, and cut a number of sections, which must be received in warm water at 60 to 65 C.
What to read after A Course of Elementary Practical Bacteriology Including Bacteriological Analysis?
You can find similar books in the "Read Also" column, or choose other free books by A a Kanthack to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
(c) Prepare also cover-glass specimens of the spleen and peritoneal fluid, and stain them with Loffler's methylene- blue, and examine them with - in. Oil immersion.
Quick Method of Hardening and of Embedding in Paraffin (1) Place small pieces of the mouse's spleen, kidney, liver, and lung in absolute alcohol.
(2) Change the alcohol every half -hour, using always an excess.
(3) After one and a half to two hours place the small pieces of tissue in a small corked bottle containing benzol, till the...y are transparent (a few minutes).
(4) Now pour off the excess of benzol, so that the pieces are only just covered by it, add a few shavings of paraffin (56 to 60 C. Melting point), and place the bottle on the paraffin stove, till the paraffin liquefies.
134 BACTERIOLOGICAL ANALYSIS LESSON (5) Transfer the specimens to liquid hard paraffin (56 to 60 C. ), and allow them to soak for five minutes.
(6) Pour the paraffin and the tissues into a small paper box, and when the paraffin is set, cut out the pieces of tissue, fix them on the rocking microtome, and cut a number of sections, which must be received in warm water at 60 to 65 C.
What to read after A Course of Elementary Practical Bacteriology Including Bacteriological Analysis?
You can find similar books in the "Read Also" column, or choose other free books by A a Kanthack to read onlineMoreLess
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