A Course of Practical Histology

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It is difficult to induce the crystallisation in human blood ; and to obtain the crystals readily it is best to employ the blood of the dog, rat, guinea-pig, or squirrel.
If a drop of rat's blood is mixed with an equal amount of distilled water, the haemoglobin becomes extracted from the corpuscles ; until this is done no crystallisation takes place.
As the excess of water begins to evaporate small needle- shaped crystals of haemoglobin appear, either singly or in bunches, which become graduall
...y larger until they may attain a very considerable size. They are mounted by covering them in thick Canada balsam.
Another method is the following : — The animal is bled, and the blood as it flows from the divided vessels is vigorously stirred with a bundle of wires, to remove the fibrin. A small quantity of the whipped blood is then mixed with about one-third its volume of 78 PRACTICAL HISTOLOGY water, and a drop of chloroform ' being added, the mixture is thoroughly shaken up for a minute or two.


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