A Course of Practical Histology Being An Introduction to the Use of the Microsc

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A Course of Practical Histology Being An Introduction to the Use of the Microsc
Edward Albert Sharpey Schfer
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In most of the lower animals they must be sought much higher in the abdomen than in the human female; in the rabbit they occur as small elongated bodies, dotted all over with little projections (the Graatian follicles), and situated just below the kidneys. They are left in the chromic solution for seven days, and then placed in spirit, and in two or three days more are ready for cutting. The hardening is effected still more readily by using the chromic and spirit mix- ture. The sections are to ...be stained in carmine solution ; for logwood sometimes colors very deeply the coagulated fluid in the Graafian follicles, so that the epithelial contents are obscured ; this coloration is probably owing to the presence of mucus (or rather THE OVUM. 239 mucin) in the secretion of the follicle. After having been stained the sections are passed through alcohol and oil of cloves, and mounted in dammar.
Preparation 9. The ovum. The ripe mam- malian ovum, although it can be seen within the larger Graafian follicles in the sections of the hardened organ, forms a much more beautiful ob- ject when obtained isolated from the ovary of a recently-killed animal.


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