A Course of Practical Physiology for Agricultural Students

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(Use human urine.) Treat 50 c.c. of urine with two drops of ammonia and saturate the solution with powdered ammonium chloride. Allow excess of ammonium chloride to settle and pour off the supernatant liquid into another beaker. The liquid contains a gelatinous precipitate of ammonium urate. Filter off the precipitate and transfer it to an evaporating dish. Add two or three drops of strong nitric acid, and evaporate to dryness on a water bath. A pink residue is left which becomes purple on the a...ddition of dilute ammonia solution.
5—2 72 PRACTICAL PHYSIOLOGY (7) To demonstrate the presence of indican. (Use human and horse's urine.) Treat 5 c.c. of urine with slightly larger volume of concentrated HC1 and about 2 c.c. of chloroform. Add a drop of 3 per cent, potas- sium chlorate solution and shake. Allow chloroform to settle and examine its colour. Blue indicates presence of indican. If still colourless add another drop of chlorate solution. If no blue colour be found in the chloroform indican is absent.


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