A Cytological Study of Artificial Parthenogenesis in Strongylocentrotus Purpurat

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A Cytological Study of Artificial Parthenogenesis in Strongylocentrotus Purpurat
Edward Hindle
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During the metaphase each chromosome splits longitudinally, and in the succeeding anaphase the halves move away from each other along the spindle fibres, 18 going to each pole (Fig. 7). As they approach the centrosomes the chromosomes swell up and become indistinct, and finally fuse together to form two daughter nuclei. These changes are accompanied by a division of the cytoplasm into two equal parts, followed by complete disappearance of the spindle fibres, and the first cleavage is complete (
...Fig. 8).
Each daughter nucleus remains partially surrounded by a clear region that seems to represent the centrosome together with a slight accumulation of cytoplasm, and here the cleavage aster of the next division first makes its appearance. After the first cleav- age is complete the succeeding divisions follow each other in re- gular succession and present all the features of ordinary mitosis (Fig. 9).
In each case the nucleus becomes completely surrounded by a clear zone of hyaloplasm. This is followed by a period of nuclear growth during which this perinuclear zone almost disappears.


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