A Guide to the Microscopic Examination of the Eye
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Orange G. — An anilin dye. A concentrated aqueous solution is prepared, and used diluted with water or alcohol.
3. Aurantia is employed in the same manner.
4. Acid-Fuchsin. — This is used like the others of the series, but, unlike them, is not removed by wash- ing, so that care must be taken not to overstain.' Triple Staining.
I. Van Gieson's Stain: — 1. Hardening in Miiller's fluid or otherwise.
2. Imbedding in celloidin. Section-cutting.
3. Preliminary staining in Delafield's or Boehmer's 62 ...GUIDE TO THE MICROSCOPIC hasmatoxylin. It is better to overstain the sections (half an hour), and not, as was originally recommended, to keep them in the hasmatoxylin for only 3-5 minutes.
4. Thorough washing in water.
5. Staining for a minute or two in a mixture consist- ing of a saturated aqueous solution of picric acid to which a few drops of a saturated aqueous solution of acid-fuchsin are added before use. The depth of the fuchsin stain may, if necessary, be lessened by immers- ing the sections in a solution of picric acid.
What to read after A Guide to the Microscopic Examination of the Eye?
You can find similar books in the "Read Also" column, or choose other free books by Greeff, R. (Richard), 1862-1938 to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Orange G. — An anilin dye. A concentrated aqueous solution is prepared, and used diluted with water or alcohol.
3. Aurantia is employed in the same manner.
4. Acid-Fuchsin. — This is used like the others of the series, but, unlike them, is not removed by wash- ing, so that care must be taken not to overstain.' Triple Staining.
I. Van Gieson's Stain: — 1. Hardening in Miiller's fluid or otherwise.
2. Imbedding in celloidin. Section-cutting.
3. Preliminary staining in Delafield's or Boehmer's 62 ...GUIDE TO THE MICROSCOPIC hasmatoxylin. It is better to overstain the sections (half an hour), and not, as was originally recommended, to keep them in the hasmatoxylin for only 3-5 minutes.
4. Thorough washing in water.
5. Staining for a minute or two in a mixture consist- ing of a saturated aqueous solution of picric acid to which a few drops of a saturated aqueous solution of acid-fuchsin are added before use. The depth of the fuchsin stain may, if necessary, be lessened by immers- ing the sections in a solution of picric acid.
What to read after A Guide to the Microscopic Examination of the Eye?
You can find similar books in the "Read Also" column, or choose other free books by Greeff, R. (Richard), 1862-1938 to read onlineMoreLess
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