A Handbook for the Physiological Laboratory, Containing An Exposition of the ...
A Handbook for the Physiological Laboratory, Containing An Exposition of the ...
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Add alcohol to the filtrate till glycogen begins to be copiously' precipitated, taking care not to add an excess of alcohol, lest other substances be also precipitated. The glycogen is l>est precipitated when the mixr ture contains 60 per cent, of absolute alcohol. Collect the gly- cogen in a filter, wash it, first with dilute alcohol, then with strong alcohol of 90 per cent. (sp. gr. 822), which makes it more easy to separate from the filter. Extract it with ether and dry it rapidly on a flat ...plate. Instead of separating the albumin from the glycogen by potassio-mercuric iodide, the boiling solu- tion of glj'cogen may be slightly acidulated with acetic acid and filtered. The filtrate is then quickly evaporated to half its bulk and mixed with its own volume of strong alcohol of 90 per cent. The glycogen is precipitated along with a little glu- tin. To separate it fiom this it is boiled with liquor potassse for an hour or more, neutralized with acetic acid, precipitated with alcohol, collected on a filter, washed first with strong alco- hol and then with absolute alcohol till all traces of water have been removed, and then the alcohol displaced by absolute ether.
What to read after A Handbook for the Physiological Laboratory, Containing An Exposition of the ...?
You can find similar books in the "Read Also" column, or choose other free books by Sir John Burdon-Sanderson, Edward Klein to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Add alcohol to the filtrate till glycogen begins to be copiously' precipitated, taking care not to add an excess of alcohol, lest other substances be also precipitated. The glycogen is l>est precipitated when the mixr ture contains 60 per cent, of absolute alcohol. Collect the gly- cogen in a filter, wash it, first with dilute alcohol, then with strong alcohol of 90 per cent. (sp. gr. 822), which makes it more easy to separate from the filter. Extract it with ether and dry it rapidly on a flat ...plate. Instead of separating the albumin from the glycogen by potassio-mercuric iodide, the boiling solu- tion of glj'cogen may be slightly acidulated with acetic acid and filtered. The filtrate is then quickly evaporated to half its bulk and mixed with its own volume of strong alcohol of 90 per cent. The glycogen is precipitated along with a little glu- tin. To separate it fiom this it is boiled with liquor potassse for an hour or more, neutralized with acetic acid, precipitated with alcohol, collected on a filter, washed first with strong alco- hol and then with absolute alcohol till all traces of water have been removed, and then the alcohol displaced by absolute ether.
What to read after A Handbook for the Physiological Laboratory, Containing An Exposition of the ...?
You can find similar books in the "Read Also" column, or choose other free books by Sir John Burdon-Sanderson, Edward Klein to read onlineMoreLess
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