A Laboratory Course in Serum Study Bacteriology 208 Being a Series of Experime

Cover A Laboratory Course in Serum Study Bacteriology 208 Being a Series of Experime
A Laboratory Course in Serum Study Bacteriology 208 Being a Series of Experime
Hans Zinsser
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2 units 18 0. 2 c. C. 2 units Syphilitic (++++) Serum 19 0. 1 c. C. 2 units 20 0. 2 c. C. 2 units The volume is made to 2 c. C. As nearly as possible in all the tubes.
All tubes are put in water bath for one hour. Then 0. 5 c. C. Of 5 per cent sheep cells and two units of amboceptor are added to each tube (ex- cept 2 tube 7) and the tubes are all returned to the water bath for thirty minutes.
CONCLUSIONS TO BE DRAWN FROM THE ABOVE Series 1 The anticomplementary dose is that quantity of antigen
...which shows the slightest inhibition of laking. The amount of antigen used in the test must under no circumstances exceed one half of this amount and is usually taken as one third of it. Thus if Tube 5 containing 1. 6 c. C. Of the 1 to 10 antigen shows a faint haze of undissolved red cells, then the quantity used in the tests should not exceed 0. 5 c. C.
Series 2 An antigen which is haemolytic in itself in Tube 7 is rejected as useless. When the extractions are made of normal heart this occurrence is rare.


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