A Laboratory Guide in Bacteriology for the Use of Students Teachers And Practi
A Laboratory Guide in Bacteriology for the Use of Students Teachers And Practi
Paul Gustav Heinemann
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Also make a stained preparation from the heart's blood, and note the typical polar staining. SECTION 8 THE ANTHRAX GROUP Members Bacillus anthracis. B. Subtilis. Great caution is necessary in handling B. Anthracis. Inoculate agar slants from laboratory cultures of B. Anthracis and B. Subtilis. 128 LABORATORY GUIDE IN BACTERIOLOGY 1. Routine study. Observe the colonies formed on agar plates. Also prepare gelatin plates and study the colonies. 2. Special study. Make an "impression prepara tion" (...Klatschpraparat) from a surface colony on a gelatin plate. Method a) Clean and flame a cover slip. b) Place on a colony of suitable size, and gently press down, taking care not to press so hard as to disturb the characteristic shape of the colony. c) Lift the cover slip with the forceps. d) Dry, fix, and stain with methylene blue or by Gram's method. e) Examine under low and high power (dry lens). 3. Special study. Staining of spores. Moller's method: a) Prepare several (five or six) films from agar or potato cultures of B.
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