A Laboratory Guide in Practical Bacteriology, With An Outline for the Clinical Examination of the Urine, Blood And Gastric Contents

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Either method usually shows both the fibrin net work andPneumoccocci well, when examined with the -^ oil immersion lens.
Staining of Tissues for Pyogenic Micrococci.
6. Stain sections of erysipelas of rabbit's ear.
(a) By Gram's method, couuterstaining in eosin (page 18).
(6) By Gram's method, previously staining in picro- carmine (page 19).
7. Stain the sections of kidney, from acute suppura- tive nephritis. (Staphyloocus pyogenes aureus).
(a) With eosin and methylene blue (page 21).
(?)), By
...Gram's method, previously staining with picrocarmine or couuterstaining in eosin.
These methods may be employed for other tissues, containing the pyogenic micro-organisms.
TUBERCULAR SPUTUM. 31 DEMONSTRATION XII.
Pure Cultures of Tubercle Bacillus — Tubercular Sputum.
1. From the glycerine agar cultures of the Tubercle bacillus prepare cover glass preparations, and stain in the following manner : (a) Float in Ziehl's carbol fuchsin for 5 minutes.
(6) Wash in water and then rinse quickly through 33^% nitric acid solution.


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