A Laboratory Manual of Physiological And Clinical Chemistry And Toxicology
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— 1 c.c. mercuric nitrate = .010 Gives amount of urea in 10 Of Physiological Chemistry 55 c.c. urine. Calculate entire amount for 24 hours.
Normal amount, 25 to 40 grammes in 24 hours.
Uric Acid (Qualitative).
Take 20 c.c. urine and add to it 2 c.c. cone.
HC1. Set aside for 24 hours in a cool place.
The uric acid which has separated out is coU lected on a filter and subjected to the murexid test.
Murexid Test. — Place the crystals in an evaporating dish, and dissolve them in a few drops of HNO3... with the application of heat.
Evaporate carefully to dryness, when a yellow- ish-red spot will be found to remain. Cool, and add a drop of ammonia, when a beautiful pur- plish-red color will develop, owing to the formation of ammonium purpurate (murexid) .
Quantitative (Hopkins' Method) .
Take 100 c.c. urine and add 30 grammes of finely powdered ammonium chloride. The mixture is then allowed to stand for two hours, and is stirred from time -to time. Then filter and wash the pp. two or three times with a satu- rated solution (NH 4 )C1.
What to read after A Laboratory Manual of Physiological And Clinical Chemistry And Toxicology?
You can find similar books in the "Read Also" column, or choose other free books by Austin, Arthur Everett, 1861- to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
— 1 c.c. mercuric nitrate = .010 Gives amount of urea in 10 Of Physiological Chemistry 55 c.c. urine. Calculate entire amount for 24 hours.
Normal amount, 25 to 40 grammes in 24 hours.
Uric Acid (Qualitative).
Take 20 c.c. urine and add to it 2 c.c. cone.
HC1. Set aside for 24 hours in a cool place.
The uric acid which has separated out is coU lected on a filter and subjected to the murexid test.
Murexid Test. — Place the crystals in an evaporating dish, and dissolve them in a few drops of HNO3... with the application of heat.
Evaporate carefully to dryness, when a yellow- ish-red spot will be found to remain. Cool, and add a drop of ammonia, when a beautiful pur- plish-red color will develop, owing to the formation of ammonium purpurate (murexid) .
Quantitative (Hopkins' Method) .
Take 100 c.c. urine and add 30 grammes of finely powdered ammonium chloride. The mixture is then allowed to stand for two hours, and is stirred from time -to time. Then filter and wash the pp. two or three times with a satu- rated solution (NH 4 )C1.
What to read after A Laboratory Manual of Physiological And Clinical Chemistry And Toxicology?
You can find similar books in the "Read Also" column, or choose other free books by Austin, Arthur Everett, 1861- to read onlineMoreLess
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