A Laboratory Manual of Physiological And Pathological Chemistry for Students in

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In regard to the addition of the mercury solution, the specific gravity serves as a guide in the case of normal urines. The number of cubic centimeters of the mercury solution to be added at first is the same as the last two figures of the specific gravity (10 cc. If the specific grav- THE URINE. 183 ity is 1. 010). The number of cubic centimeters of the mer- cury solution used to obtain the end-point of the reaction expresses the amount of the urea in the urine in grams per liter. If considera...bly less than 30 cc. Is found necessary to- obtain the end-point, a correction (according to Liebig) must be applied. The difference between 30 and the number of cubic centimeters actually used is divided by 5. This num- ber represents the tenths of cubic centimeters which must be subtracted from the number of cubic centimeters actually used. The entire determination is to be made at least twice on the same urine. It is also advisable to make a determination with a fever urine, in which case the detection of the end-reaction is more difficult, and the greater concentration of the urine may also cause difficul- ties (in the case of very concentrated urine take an equal quantity of urine and the baryta mixture ; 15 cc.

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