A Laboratory Manual of Physiological Chemistry

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and weigh. Calculate amount of kreatinin from the weight of the salt.
5. Coloring matter of normal urine. — The yellow color of normal urine depends apparently upon several coloring matters not yet isolated (called by Garrod urochrom). Several pig- ments, however, appear in urine in conditions which are more or less normal. These pigments are derived from the biliary color- The Chemistry of the Excretions 75 ing matters (see table under "Blood," chap. 4). Among these pigments we have urobilin,
...uroerythrin, and urobilinoidin.
a) Preparation XXXI: Preparation of urobilin (Jaffe).
— Add to the urine a solution of basic lead acetate, filter, wash the precipitate with water, dry at ordinary tem- perature, boil it with alcohol, and decompose it, when cold, with alcohol containing sulphuric acid. The filtered solu- tion is diluted with water, saturated with ammonia, and then treated with zinc chloride solution. Wash this lat- ter precipitate free from .chlorine by water, boil it with alcohol, dry, dissolve in ammonia, and precipitate this solution with lead acetate.


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