A Laboratory Manual of Physiological Chemistry

Cover A Laboratory Manual of Physiological Chemistry
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Below it is bent upward aiid expands to a bulb. The graduations represent for each division 0.001 gramme of urea. That is, 0.001 gramme of urea evolves enough nitrogen to fill one division. Since one cubic centimeter of urine is used, weighing very nearly a gramme, nitrogen to fill one division corresponds very nearly to 0.1 per cent, of urea in the urine. With the tube is furnished a l-cubic-eentimeter dropping pipette.
An apparatus can be simply and cheaply made, after the principle of Squibb
...'s, from two 4-ounee wide-mouth bottles (see figure). One of these (A) contains a vial (C), which serves to hold the urine.
AMOUNT OF TJEEA.
135 Outside C, in .-I, is placed the solution of sodium hy- pobromite. B contains water and is connected with A by a rubber tube. "\Mien the rubber stoppers are tightly in- serted the urine is brought into contact with the hypo- bromite by tipping A, the nitrogen of the urea being liberated. This forces from B an equal volume of water.
The water is collected from the tube D, and when meas- xued gives the volume of nitrogen set free.


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