A Manual of Elementary Microscopical Manipulation for the Use of Amateurs
A Manual of Elementary Microscopical Manipulation for the Use of Amateurs
Thomas Charters White
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. '2 ounces. Distilled water . . . . 2 Alcohol . . . . . . ^ ounce. " The carmine in small fragments is to be placed in a test tube, and the ammonia added to it. By agitation, and with the aid of the heat of a spirit lamp, the carmine is soon dissolved. The ammonical solution is to be boiled for a few seconds, and then allowed to cool. After the lapse of an hour, much of the excess of ammonia will have escaped. The glycerine and water may then be added, and the whole passed through a filter or ...allowed to stand for some time, and the perfectly clear supernatant fluid poured ofl" and kept for use. This solution will keep for months ; but sometimes a little carmine will be deposited owing to the escape of ammonia, in which case one or two drops of liquor ammonia to the four ounces of carmine solution may be added. " Logwood stain may be made so easily from any of the numerous formula given in the text books, that it may seem unnecessary to unduly burden this small Manual by giving one : the desire of the author being to furnish the student with as much elementary information as he can, which, while condensed, will yet be clear and serviceable to him.
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