A Practical Text book of Infection Immunity And Specific Therapy With Special
A Practical Text book of Infection Immunity And Specific Therapy With Special
John Albert Kolmer
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6 Berl. Klin. Wochenschr. , 1911, 43, 1935. 524 THE RELATION OF COLLOIDS AND LIPOIDS TO IMMUNITY Five constituents enter into the test: 1. The Antigen. This is an alcoholic extract of syphilitic liver, prepared in exactly the same manner as for performing the Wassermann reaction. High dilutions of the antigen, ranging from 1 : 100 to 1 : 10, 000, are prepared with normal salt solution. As in the Wassermann re- action, not every antigen is satisfactory, a point that can be determined only by mak...ing preliminary tests. 2. The patient's serum should be fresh, unheated, and highly diluted, the dilutions ranging from 1 : 100 to 1 : 10, 000, 000. Usually it is better to use higher than lower dilutions. When too concentrated solutions of serums and of antigen are used, erroneous results are likely to be ob- tained. 3. A normal solution of sulphuric acid. 4. A saturated solution of barium hydroxid made equivalent to the normal solution of sulphuric acid. In the use of the barium hydroxid it is imperative to prevent its exposure to the air.
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