A Review of the Carbohydrate Constituents of Roughages
A Review of the Carbohydrate Constituents of Roughages
R Gaurth Roger Gaurth Hansen
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Tracey (121) described a colorimetric method for the estimation of pentoses in the presence of large amounts of hexoses and uronic acids. This is accomplished by allowing the reaction to take place at room temperature and taking advantage of the fact that pentoses, especially xylose, react faster. Factors influencing color development, nature of the reaction, sensitivity, and specificity are given. Difficulty can still be encountered, however, when using this method for the estimation of cell-w...all polysaccharides as it is not completely specific. McRary and Slattery (79) outlined a colorimetric determination of pentoses and pentosans which has the same limitations as other tests in reference to accuracy of estimation of the material present in hemicelluloses. Estimation of Uronic Acids A number of methods have been described for the determination of uronic acids. Most of them depend on the fact that the acid may be more or less quantitatively decarboxylated when heated with suffi- ciently concentrated mineral acids and the carbon dioxide liberated can be determined as a measure of the uronic acid, free or combined, originally present.
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