A Review of the Literature of Phosphorus Compounds in Animal Metabolism

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Osborne and Mendel (191 la) in their first year's investigation on feeding with isolated food substances (reported in Carnegie In- stitution Publication 156, Part I) conducted many experiments in the feeding of white rats on rations of pure casein, cane sugar, starch, lard, agar and inorganic salts. Varying results, as deter- mined by the salt mixtures, were obtained. Weight was main- tained and nitrogen stored over considerable periods of time with both young and mature rats, but without signi
...ficant gain in weight, on rations containing but a single protein. One animal was main- tained for 217 days on a diet in which the sole protein was glutenin.
In the second year's work, as reported in Part II of the above- mentioned publication, a large number of pure proteins were com- pared, some containing phosphorus, and others free from this ele- ment. A noteworthy change of method was the addition to the ration of "protein-free" milk, a preparation made from skim milk by precipitation of the casein with hydrochloric acid, filtration, and evaporation of the whey.


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