A Stanford Professors Career in Biochemistry Science Politics And the Biotech

Cover A Stanford Professors Career in Biochemistry Science Politics And the Biotech
A Stanford Professors Career in Biochemistry Science Politics And the Biotech
Paul Berg
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More on Recombinant DNA Science Construction of Recombinant DNA Molecules to Study the Mammalian Cell Berg: So we said, okay, that s not going to work. Can we construct DNA molecules which in fact use the virus chromosome? Let s just attach foreign pieces of DNA, any genes we might ultimately want, to the viral DNA. At the time, you have to remember, nobody had isolated any genes. I believed that that would eventually be 87 achieved. People would find ways. One hint that this was likely was tha
...t certain classes of genes have physical properties which allowed them to be separated from the bulk of cellular DNA.
Don Brown, at the Carnegie Labs in Baltimore, was able to isolate pure ribosomal RNA genes, because they have an unusual buoyant density. That is, if you put them in a centrifuge in a gradient of salt concentration, the ribosomal DNA genes separate from the rest of the DNA. And, you can purify them that way. Nobody had isolated any other genes, but it wasn t too far-fetched that there would be a way to isolate individual genes sometime in the future.


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