A Study of Epithelioma Contagiosum of the Common Fowl
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The antigen was found no longer to prevent haemolysis at a dilution of 1 to 100, so was used at a dilution of 1 to 200. The alexin usually produced complete haemolysis at a dilution of 1 to 20 or 1 to 25. The amboceptor was used at a dilution of 1 to 1000, and the sheep cells in a 5% suspension in physiological salt solution.
Complement Fixation.- — With normal serum in quantities from 0.1 ccm. up to 0.8 ccm. haemolysis was complete. Haemolysis was usually complete after two hours, and always a...t the end of five hours' incubation. The same result was obtained with serum taken from infected fowls during the period of incubation. How- ever, a different result was obtained with serum from fowls in which the lesions of epithelioma contagiosum were present. In this serum there was considerable evidence of a specific antibody.
Haemolysis did not occur as readily and was almost constantly incomplete at the end of ten hours incubation. The results were not as striking as we believe they could be made by variation in the nature of the antigen, and since there is no question of diagnosis involved will never be of practical value, but it is cer- 46 University of California Publications in Zoology V^^^- H tainly of interest as denoting the formation of a specific antibody, or a deviation of complement, in the serum of the infected fowl by an infection of epithelioma contagiosum.
What to read after A Study of Epithelioma Contagiosum of the Common Fowl?
You can find similar books in the "Read Also" column, or choose other free books by Sweet, Clifford D. (Clifford Daniel), 1885- to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
The antigen was found no longer to prevent haemolysis at a dilution of 1 to 100, so was used at a dilution of 1 to 200. The alexin usually produced complete haemolysis at a dilution of 1 to 20 or 1 to 25. The amboceptor was used at a dilution of 1 to 1000, and the sheep cells in a 5% suspension in physiological salt solution.
Complement Fixation.- — With normal serum in quantities from 0.1 ccm. up to 0.8 ccm. haemolysis was complete. Haemolysis was usually complete after two hours, and always a...t the end of five hours' incubation. The same result was obtained with serum taken from infected fowls during the period of incubation. How- ever, a different result was obtained with serum from fowls in which the lesions of epithelioma contagiosum were present. In this serum there was considerable evidence of a specific antibody.
Haemolysis did not occur as readily and was almost constantly incomplete at the end of ten hours incubation. The results were not as striking as we believe they could be made by variation in the nature of the antigen, and since there is no question of diagnosis involved will never be of practical value, but it is cer- 46 University of California Publications in Zoology V^^^- H tainly of interest as denoting the formation of a specific antibody, or a deviation of complement, in the serum of the infected fowl by an infection of epithelioma contagiosum.
What to read after A Study of Epithelioma Contagiosum of the Common Fowl?
You can find similar books in the "Read Also" column, or choose other free books by Sweet, Clifford D. (Clifford Daniel), 1885- to read onlineMoreLess
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