A Study of the Decomposition Products of Spore Bearing Bacteria in Heated Milk

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A Study of the Decomposition Products of Spore Bearing Bacteria in Heated Milk
Henrietta Lisk
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Reducing substances calculated as lactose were decreased in two eight day cultures but increased in a third. In twenty days a decrease was noted.
60 Total acidity .
In eight day cultures a decrease of 0, 2 co, 0. 6 cc. And 8, 2 co. N/10 acid per 100 cc of the medium was found, but in twenty days an increase of l#6 co K/10 aoid per 100 co of the medium -was obtained in "reaction".
Hydrogen ion concentration decreased in eight days from 0. 2 to 0. 3 and in twenty days decreased 1. 2.
Volatile aci
...d.
Volatile acid content of eight day cultiires varied from no change from the original medium to 0. 6 H/IO acid cc. Per 100 cc of the medium. In twenty days 0. 8 cc K/IO acid per 100 cc of medium were formed.
Acetic acid was present in the distillate Txit not in sufficient amounts to distinguish tl» culture from the original medium. Indole. Evdrogen sulT)hide. And Meroaptan. Ko indole, hydrogen sulphide or me reap tan foitoation could be demon- strated by the action of Bacillus simplex.
TABLE IX BACILLUS SIMPLEX* Days of prowth 8 20 A B G D t Nitropen as anmonla 0.


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