A Study of the Initial Velocity in the Hydrolysis of Sucrose By Invertase

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F. M. Beegle. Beegle permitted a 5% cane sugar solu- tion (B) to hydrolyze completely with invertase, then added 5 grms. More of finely powdered (so that solution would take /*r ^ ^ ^ ^ / = Part A " > GJ " A -Ex per in A - d- ent L Z I JT ^/ Y 30 60 90 120 150 ISC Minutes Fro!
19 place as rapidly as possible) cane sugar per 100 cc. Of the solu- tion and allowed the hydrolysis to continue. The velocity hy- drolysis of the added portion of cane sugar was then compared with the velocity of a 10% c
...ane sugar solution (A) after the latter had passed the 50% hydrolyzed stage. The change in rotation of the solutions and the corresponding times are given in Table IV, and the corresponding graphs in Figure III.
The calculated rotation of the 10% cane sugar solution A when 50% hydrolyzed was 3. 80. The rotations given in Table IV were determined by adding 5 cc. Of a sodium car- bonate solution to 20 cc. Of the sugar solution and then ex- amining it in the polariscope with a 200 mm. Tube. The inver- tase correction for the rotations in this set of experiments was negligible.


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