A Text book of Bacteriology a Practical Treatise for Students And Practitioners

Cover A Text book of Bacteriology a Practical Treatise for Students And Practitioners
A Text book of Bacteriology a Practical Treatise for Students And Practitioners
Philip Hanson Hiss
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Doses from 2 to 5 c. C. Should be given. In giving the later injections the danger of anaphylaxis must be remem- 1 R. Kraus, Wien. Klin. Woch. , 1897; Norris, Jour. Inf. Dis. , 1 and 3, 1904. 2 Myers, Lancet, ii, 1900. 3 Norris, loc. Cit.
54 INFECTION AND IMMUNITY bered. A single injection of a large quantity has occasionally yielded a precipitating serum of considerable strength, 1 but this method is not usually successful. Injections are made at intervals of from five to seven days. Seven to
...twelve days after the last injection the animals may be bled from the ear, and a preliminary test made to ascertain the precipitating value of the serum. If this is insufficient, more injections may be made. Bleeding should be done 7 to 12 days after the last injection. Such sera may be preserved in the dark and at a low tem- perature. If a preservative is added, Nuttall prefers chloroform to the phenols, because of occasional turbidity produced by these.
Precipitating antisera for tests should be clear.


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