A Text-Book of Physiological Chemistry for Students of Medicine

Cover A Text-Book of Physiological Chemistry for Students of Medicine
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of the liquid make the albumose test. Add to it finely powdered zinc sulphate as long as it dissolves on stirring. On saturating the solution completely a flocculent precipitate gradually settles. This is essentially the " albumose " frac- tion and may contain a little gelatin. After 24 hours filter, and test the filtrate for peptone by the biuret reaction ; this is generally negative.
Use the remainder of the original solution for the recognition of creatine. Add to it carefully a solution of
...basic acetate of lead as long as a precipitate forms.
This will carry down phosphates, sulphates and other compounds forming insoluble combinations with it, but not creatine. A slight excess of the lead must be added to insure complete precipitation. This can be determined by allowing the first formed precipitate to settle and adding more reagent as necessary. Finally filter, and remove the excess of lead by passing in hydrogen sulphide. Filter again, and remove as much as possible of the excess of sulphide used, by shaking.


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