Agricultural Bacteriology Theoretical And Practical
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' . 100 c. C. Lemco's beef extract . . , 5 gr. Witte's peptone . . . I. O gr. Urea . . ... . 10. 0 gr.
Boil ; cool. The medium should be slightly alkaline ; if not, add a drop or two of ammonium carbonate solution. Place in a 250 c. C. Flask and infect with a gram of garden soil, and incubate at 20 to 30 C. For ten days.
(a) Examine a drop of the fermenting medium from day to day, and determine if cocci or bacilli are present. Stain with gentian violet. At first Urococci or Planosardna may be p...resent, 224 FARMYARD MANURE but later an almost pure culture of Urobatillus Pasteurii is obtained, the large amount of urea (10 per cent. ) inhibiting the growth of most of the other organisms met with in the soil.
(b) Transfer a drop to a fresh flask of the medium, incubate for ten days at 30 C. , and examine as before.
(c) Repeat the above, using garden soil which has been heated to 90 C. For one hour instead of a fresh sample.
Ex. 104. Prepare 250 c. C. Urea-bouillon as above; infect with garden soil and incubate at 22 C.
What to read after Agricultural Bacteriology Theoretical And Practical?
You can find similar books in the "Read Also" column, or choose other free books by Percival John to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
' . 100 c. C. Lemco's beef extract . . , 5 gr. Witte's peptone . . . I. O gr. Urea . . ... . 10. 0 gr.
Boil ; cool. The medium should be slightly alkaline ; if not, add a drop or two of ammonium carbonate solution. Place in a 250 c. C. Flask and infect with a gram of garden soil, and incubate at 20 to 30 C. For ten days.
(a) Examine a drop of the fermenting medium from day to day, and determine if cocci or bacilli are present. Stain with gentian violet. At first Urococci or Planosardna may be p...resent, 224 FARMYARD MANURE but later an almost pure culture of Urobatillus Pasteurii is obtained, the large amount of urea (10 per cent. ) inhibiting the growth of most of the other organisms met with in the soil.
(b) Transfer a drop to a fresh flask of the medium, incubate for ten days at 30 C. , and examine as before.
(c) Repeat the above, using garden soil which has been heated to 90 C. For one hour instead of a fresh sample.
Ex. 104. Prepare 250 c. C. Urea-bouillon as above; infect with garden soil and incubate at 22 C.
What to read after Agricultural Bacteriology Theoretical And Practical?
You can find similar books in the "Read Also" column, or choose other free books by Percival John to read onlineMoreLess
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