An Introduction to Chemical Pharmacology: Pharmacodynamics in Relation to ...
An Introduction to Chemical Pharmacology: Pharmacodynamics in Relation to ...
Mcguigan, Hugh, 1874-
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water and shake thoroughly. Pour 100 grams of the clear ether extract into another flask and add 40 cc. of 1 per cent, sulphuric acid. Shake thoroughly and allow to settle. The acid aqueous solution contains the alkaloidal sulphates. Pour off most of the ether without losing any of the water solution. Transfer the acid solution to a separatory funnel and make alka- line with ammonium hydroxide (6 cc. 10 per cent, solution). Extract with a mixture of yi ether and % chloroform, using about 40 cc.... of the mixture. Separate this extract and transfer it to a dry flask. Repeat the extraction with 20 cc. of the ethe^ chloro- form mixture. Separate and transfer this also to the flask con- taining the first extract. To get rid of the water filter through a dry filter into a weighed dry flask and allow to evaporate. The Digiti zedbyGoOgte ISOQUTNOHNE 261 alkaloids will crystallize out. After the solvent has evaporated, weigh and calculate the percentage of alkaloids in the bark. Tests for Quinine A solution of quinine in sulphur, acetic or tartaric acids shows a beautiful light blue fluorescence.
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