Analysis of Path Based Approaches to Genomic Physical Mapping
Analysis of Path Based Approaches to Genomic Physical Mapping
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A simple way to estimate the proportion of false connections is to consider loci separated by >50 cM and connected by short paths. Such paths must surely be spurious inasmuch as the average YAC length is 1 Mb, corresponding to only about 1 cM in the human genome. The proportion of such widely-separated loci pairs connected by chromosomally allowable paths of length 1, 3, 5, and 7 is 0. 05, 4, 33, and 63%, respectively. In particular, the curve rises dramatically for path lengths exceeding four,... indicating that random connections dominate at these distances. The proportion of connected loci pairs at distances 5-10, 10-20, and 20-50 cM was not much higher than for loci pairs at distances > 50 cM. This suggests that many of these paths are also spurious, and that such spurious paths span close loci pairs at approximately the same rate as distant loci pairs.
We attempted to estimate the proportions of valid paths. We partitioned the pairs of loci into six groups on the basis of their inter-locus distance: the groups consisted of loci at 0-2, 2-5, 5-10, 10-20, 20-50, and > 50 cM, respectively.
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To quickly assess the difficulty of the text, read a short excerpt:
A simple way to estimate the proportion of false connections is to consider loci separated by >50 cM and connected by short paths. Such paths must surely be spurious inasmuch as the average YAC length is 1 Mb, corresponding to only about 1 cM in the human genome. The proportion of such widely-separated loci pairs connected by chromosomally allowable paths of length 1, 3, 5, and 7 is 0. 05, 4, 33, and 63%, respectively. In particular, the curve rises dramatically for path lengths exceeding four,... indicating that random connections dominate at these distances. The proportion of connected loci pairs at distances 5-10, 10-20, and 20-50 cM was not much higher than for loci pairs at distances > 50 cM. This suggests that many of these paths are also spurious, and that such spurious paths span close loci pairs at approximately the same rate as distant loci pairs.
We attempted to estimate the proportions of valid paths. We partitioned the pairs of loci into six groups on the basis of their inter-locus distance: the groups consisted of loci at 0-2, 2-5, 5-10, 10-20, 20-50, and > 50 cM, respectively.
What to read after Analysis of Path Based Approaches to Genomic Physical Mapping?
You can find similar books in the "Read Also" column, or choose other free books by Alan Rimm Kaufman to read onlineMoreLess
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