Annual Report of the Supervising Surgeon General of the Public Health And Marine-Hospital Service of the United States V.33

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— The point is immersed in 1 e. e. of physiological salt solution for fifteen minutes to soften the vaccine matter. It is then scrai)ed off with a sterilized platinum scraper and thoroughly agitated by blowing in and out of a sterilized pipette This enudsion is then put in three Petri dishes, as follows: 5 drops in the first, 10 droi)s in the second, and the remainder in the third.
Then melted iigar is poured over them and they are incubated at .37° C. for three days, then at room temperature f
...or two days. The numi)er of colonies is then counted. When the number is small per plate, the entire number is counted. In the case of vaccine in the capillary tubes, the contents of the tubes are iilanted in 1 c. c. physiologicaT salt solution, and then thoroughly agi- tated to break up the clumps, as in the case of the point. After the plates have been incubated the various colonies are examined, and in this way we deter- mine the various kinds of contaminating organisms. The pus cocci appear to pi'edominate in all vaccine, though many contain " spreader," which often causes trouble in counting the plates.

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