Applied Bacteriology An Introductory Handbook

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Micrococcus aqilis ... 20 *" J) M Tijplioid bacillus ... 22 73-77 T. -. -7 " " " " " Uacillus subtilus ... ... 29 Bacillus cedematis maliani 36 D -77 r " " " " " Uacillus of symptomatic ) anthrax ...... .. ) "^ " " The mordant, with the proper amount of acid or alkali added, is run from a pipette on to the cover-glass, and the latter is gently warmed over a flame with constant move- ment until steam just begins to form. On no account should it be allowed to boil, for if bubbles are once formed ...STAINING OF FLAGELLA 93 the preparation is spoiled, as a fine precipitate of mordant is produced, which becomes stained later on and obscures flagella.
The heating must only last for from half to one minute ; the liquid is poured off, and the cover-glass thoroughly washed with water ; it is allowed to dry in the ordinary way. The preparation is then stained with a few drops of 5 per cent, aniline-water solution of fuchsine. The cover- glass may be gently warmed for about a minute, after which the stain is washed off very thoroughly with water ; the cover-glass is now air-dried and mounted in balsam.


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