Bacteriological Methods in Food And Drug Laboratories With An Introduction to M
Bacteriological Methods in Food And Drug Laboratories With An Introduction to M
Albert Schneider
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I cc. Quantities of the substance to be cultured, or dilutions thereof, are planted or delivered into the middle of the dish, an absolutely clean and sterile i cc, pipette accurately divided into tenths. The cover of the dish is to be lifted just high enough to permit placing the pipette in position, and is to be replaced just as soon as possible. In the usual water analysis work, i cc. Quantities are generally planted, instead of o. I cc. Quantities as above recommended. For purely quantitativ...e results, the smaller amounts should be planted because the larger amounts may include enough of the inoculating liquid to interfere with the uniformity of results. Formerly it was customary to mix the material to be planted with the medium in the tube before plating. This method has 86 BACTERIOLOGICAL METHODS some very objectionable features, chief of which is that the residue remaining in the tube after pouring retained a certain percentage of the organisms, thus interfering with the accuracy of the results.
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