Biochemic Drug Assay Methods; With Special Reference to the Pharmacodynamic Standardization of Drugs
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— Same as under Houghton's "twelve-hour" method.
Animals. — In addition to the precautions given under Houghton's "twelve-hour" method, Famulener and Lyons have suggested that, since frogs are markedly influenced by external surroundings, tempera- ture, season, relation of time of injection to time of feeding, etc., it is advisable to guard against the possibility that the frogs used for any particular series of experiments might be above or below the average susceptibility by standardizing eac...h lot before they are used for assay purposes. For this purpose most workers have adopted crystalline ouabain. When a new lot of frogs is received a test is made by in- jecting one frog with the standard "minimum dose" of ouabain, fixed by previous experiments, to another 10 per cent, more, and to a third 10 per cent, less, than the standard dose. If the result of this experiment shows that the frogs are not of normal susceptibility it is then necessary 44 BIOCHEMIC DRUG ASSAY METHODS to determine the minimum dose for this particular lot of frogs and to correct all succeeding assays accordingly.
What to read after Biochemic Drug Assay Methods; With Special Reference to the Pharmacodynamic Standardization of Drugs?
You can find similar books in the "Read Also" column, or choose other free books by Stewart, Francis Edward, 1853-1941 to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
— Same as under Houghton's "twelve-hour" method.
Animals. — In addition to the precautions given under Houghton's "twelve-hour" method, Famulener and Lyons have suggested that, since frogs are markedly influenced by external surroundings, tempera- ture, season, relation of time of injection to time of feeding, etc., it is advisable to guard against the possibility that the frogs used for any particular series of experiments might be above or below the average susceptibility by standardizing eac...h lot before they are used for assay purposes. For this purpose most workers have adopted crystalline ouabain. When a new lot of frogs is received a test is made by in- jecting one frog with the standard "minimum dose" of ouabain, fixed by previous experiments, to another 10 per cent, more, and to a third 10 per cent, less, than the standard dose. If the result of this experiment shows that the frogs are not of normal susceptibility it is then necessary 44 BIOCHEMIC DRUG ASSAY METHODS to determine the minimum dose for this particular lot of frogs and to correct all succeeding assays accordingly.
What to read after Biochemic Drug Assay Methods; With Special Reference to the Pharmacodynamic Standardization of Drugs?
You can find similar books in the "Read Also" column, or choose other free books by Stewart, Francis Edward, 1853-1941 to read onlineMoreLess
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