Biological Laboratory Methods

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A tube of sterile nutrient fluid is warmed until the fluid be- comes liquid, when it is inoculated with a trace of bacterial mix- ture by means of the sterilized pipette, and the mass is well shaken so that the bacteria are well distributed throughout the nutrient agar or gelatin. This is now quickly transferred to the bottom of a Petri dish and spread out over the surface. The Petri dish, resting on a glass plate, is at once covered by another slightly larger dish, and over these dishes is pla...ced a bell-glass jar, around the edges of which is smeared vaseline, so that the contact with the glass plate will make an air-tight connection. In the bell-glass jar is also placed dampened blotting-paper, in order that the air maybe kept in a moist condition. Of course, in all of these experiments it must be understood that all dishes and utensils used are well sterilized before the inoculation is performed. The nivellating apparatus illustrated in Fig. 109 will enable the experimenter to level the plate culture, and thus preserve a uniform depth in the nutrient fluid.

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