Cell Contact Mediated Differentiation in Dictyostelium.

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Note the layer of electron-dense substance composing the defective cell wall and the irregular cytoplasmic spaces which may have contained deposits of glycogen. M - mitochondrion, typical form found in prespore cells 30 %■ < 9'* Figure 8. Cell from the central region of the prespore mass after exposure to Con A, X26,088. Note that the cell wall is atypical in thickness and associated with the cell surface by a layer of electron-dense substance. Arrows denote mitochondria essentially similar to those observed in controls. 32 Figure 9. Cell from the prespore region after exposure to distilled water exhibiting normal cell wall, X26,088. Arrows denote mitochondria typical of normal mature spores. N - nucleus 34 P^^ii J V Figure 10. Cell from the prespore region after exposure to CAB exhibiting normal cell wall and the irregular cytoplasmic spaces which may have contained deposits of glycogen, X34,784. Arrows denote mitochondria typical of normal mature spores. 36 ••• 'ace '^v •' : DISCUSSION Cyclic AMP in Prespore Differentiation Cell differentiation in Diotyosteliim depends upon cell association.

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