Chemical Pathology Being a Discussion of General Pathology From the Standpoint

Cover Chemical Pathology Being a Discussion of General Pathology From the Standpoint
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Reduction of the temperature of plant cells to — 13° may result in a granular transformation of the cytoplasm, often with rather seri- ous structural alterations. Cytoplasm seems to be more affected than the nucleus, for mitosis may occur slowly in plant cells at — 8°, and Uschinsky ^^ noted that in animal tissues the nuclei were less af- fected by cold than the cytoplasm. Blood seems little affected by freezing temperature, for du Cornu found that dog's blood kept on ice for five to ten days c...ould be employed for transfusion without causing hemoglobinuria. Grawitz saw motion persist in human cili- ated epithelium kept for seven to nine days on ice. Ciliated epi- thelium from the mouth of the frog may survive cooling to — 90°, and frog eggs are not killed by — 60°. In many cells, however, the physical changes produced by freezing, and also bj^ the subsequent thawing, are sufficient to render them incapable of further exist- ence. -'' Cells devoid of or poor in water cannot be killed by freez- ing, hence it is probable that the currents set up about the crystals of ice in thawing, as well as the rapid contraction and expansion under the influence of the cold and the ice formation, are the cause of the effects of freezing, which, therefore, are not dependent upon chemical, but upon physical, alterations.

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