Defensive Ferments of the Animal Organism Against Substances Out of Harmony With

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It is best to use a filter pump. Only at the end is the liquid allowed to pass entirely through the filter, after which the latter is immediately placed in a vacuum exsiccator. After a day or two the peptone is absolutely dry, and may then be weighed. First, a 10 per cent, solution, in 0*9 per cent, solution of common salt, is prepared, and the deviation of rota- tion of the solution is determined. If this is more than i, the solution is diluted, until it shows a rota- tion of about o'75. The h...igher degree of rotation would not be injurious. Dilution is only effected in order to make the best use of the costly material. 14 2IO STANDARDIZATION OF PEPTONES Standardization of the Peptone. Let us assume that we have to deal with placenta peptone. This is mixed with the serum of individuals, who are certainly not pregnant, and then there should be no alteration of the original rotation. Should this not be the case, then the peptone is certainly not free from sulphuric acid or barium. With the serum of pregnant individuals a decomposition is bound to take place.

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