Directions for Laboratory Work in Physiology

Cover Directions for Laboratory Work in Physiology
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Carefully separate a portion of the nerve from the surrounding tissues without in- jury to the nerve or the blood vessels. Pass a ligature under the nerve, carrying the ends around to the front of the thigh, and tie tightly, thus in- cluding all the structures of the limb except the nerve.
Cover exposed nerve with filter paper moistened with salt solution.
Inject into the dorsal lymph sac about i cc. of a stock solution of curara. Use for this purpose a pipette with fine point and rubber bulb.
...To insert pipette, raise loose skin of back over forward part of dorsal lymph sack with forceps, and make small opening in skin with scissors. Lay the frog on a plate and cover it with moist filter paper. Ob- serve that pinching the toes of either hind leg slightly, causes a contraction of the muscles of the leg thus irritated.
As the drug takes effect, the ability of the right leg to re- Fig. 9. Relation of brain to skull of frog. A, cerebral hemi- spheres; B, eyes; C, thalamen- cephalon; D, opticlobes; E, tym- panic membrane; F, cerebellum; G, medulla oblongata; H, spinal cord.


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