Effects of Cold Temperature On Toxicity of Ammonia to Rainbow Trout, Bluegills, And Fathead Minnows : Contract Report 68-01-5832/b

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The 96-h LC50 values for cold and warm temperatures, respectively, were 0.59 and 0.97 mg/L NH3-N in test I and 0.61 and 1.36 mg/L NH3-N in test II. Corresponding LC50 values for total ammonia nitrogen were 15.28 and 17.60 mg/L in test I and 25.07 and 20.90 mg/L in test II.
Effects of ammonia on equilibrium and on feeding were also observed. At 4°C, 10% mortality occurred in 120 h at 0.17 mg/L NH3-N and another 5% showed loss of equilibrium. At 25°C, 60% mortality occurred at 1.5 mg/L NH3-N; sur
...viving fish venti- lated at an obviously higher rate than did controls and they did not feed. At 1.0 mg/L NH3- N, only 5% mortality occurred but survivors were less active and responded more slowly to food than did controls.
VI. DISCUSSION Results of this study show that rainbow trout, bluegill, and fathead minnow are more sensitive to un-ionized ammonia, as determined by mortality and by sublethal effects, at low temperatures typical of winter conditions than at higher temperatures typical of summer conditions for each species.


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