Effects of Specific Factors On the Fatty Acid Composition of Streptococcus Lactis
Effects of Specific Factors On the Fatty Acid Composition of Streptococcus Lactis
Kral, Timothy Alan
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CHAPTER V EFFECTS OF CITRATE ON THE FATTY ACID COMPOSITION OF S. lactis Literature Review In eucaryotic systems, enzymes concerned with citrate metabolism play an important role in fatty acid biosynthesis. Investigators have shown that citrate was incorporated more rapidly into fatty acids than acetate in vitro using yeast enzymes (39). Effects of citrate on fatty acid composition in a bacterium, E. coliy have also been investigated. In a medium containing 0.1 g/1 of trisodium citrate, the perc...entage of the A17:0 fatty acid was below 6.0% when the cell density was less than 1 g/1. As the cell density increased above 1 g/1, the percentage of the A17.-0 fatty acid increased and exceeded 12.0%. There was an accompanying decrease in the 16:1 content. Cells grown in the absence of citrate had a higher percentage of A17:0 fatty acid than did cells grown in its presence, provided that the cell concentration exceeded 1 g/1. One consequence of adding citrate to the medium is chelation of Mg 4 " 1 ", a cofactor required for the formation of cyclopropane fatty acid (29).
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