Elementary Exercises in Physiology

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in a 2nd, 20 cc. in a 3rd, 25 cc. in a 4th, 30 cc. in a 5th tube. Place all in a water bath at 38-40 degrees C.
Place an egg in boiling water and boil for 15 minutes, then place it in cold water. When cold, wipe it dry, remove the coagulated albumin and rub it through a No. 30 sieve.
Place I gram of this disintegrated albumin in each of the above test tubes at 38-40 degrees and shake well, to thoroughly mix, being careful not to lose any of the solution or albumin. Keep at 38-40 degrees for six
... hours, geiitly shaking the tubes every 15 minutes.
Note the time at which digestion is completed in each tube. If the pepsin is of U. S. P. strength, this should be at the end of six hours in tube i, only a few thin, insoluble flakes at most being left. From results obtained, determine the relative strength of the pepsin. How many times its own weight of freshly coagulated albumin will it digest in six hours^ 155- Valuation of Commercial Pancreatin. Dissolve .28 gm. pancreatin in loocc. tepid water and add 1.5 gm.


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