Elements of Water Bacteriology, With Special Reference to Sanitary Water Analysis

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5 gram Pepton 2. 0 grams Water 100. 0 c. C.
This is boiled, clarified, and filtered as usual, then i. O gram lactose is added, and the medium tubed and sterilized for three successive days at 100.
B. Broth.
Sodium taurocholate (pure) 0. 5 gram Pepton 2. 0 grams Glucose 0. 5 gram Water 100. 0 grams Boil, filter, and add sufficient neutral litmus, fill fer- mentation tubes, and sterilize at 100 degrees.
2 1 6 Appendix.
Litmus Solution. To one-half pound of litmus cubes add enough water to more th
...an cover; boil, and decant off the solution. Repeat this operation with successive small quantities of water until from 3 to 4 liters of water have been used and the cubes are well exhausted of color- ing matter. Pour the decantations together and allow them to settle over night. Siphon off the clear solution. Concentrate to about i liter and make the solution de- cidedly acid with glacial acetic acid. Boil down to about one-half liter and make exactly neutral with caustic soda or potash. To test for the neutral point, place one drop of N the solution in a test tube.

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