Elements of Water Bacteriology, With Special Reference to Sanitary Water Analysis

Cover Elements of Water Bacteriology, With Special Reference to Sanitary Water Analysis
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4. Titrate and record re- action of filtrate. Say fe- action + 2.2%.
5. Weigh infusion. Say 1800 gm.
6. Set infusion on water- bath, keeping temperature below 6o° C.
7. Add peptone, gm.
1%, 18 8. After ingredients are dissolved, titrate, reaction probably + 2.3 to +2.5.
9. Neutralize, method.
Fuller's Ditto.
Ditto.
Ditto.
Ditto.
Ditto.
Ditto.
Ditto and sheet gelatin 10%, 180 gm.
Ditto.
Probably + 4.0 to + 0.5.
Ditto.
Infuse lean meat 20 hours with its own weight of dis- tilled water in refriger
...ator.
Say 1000 gm. meat.
" 1000 gm. water.
Ditto.
i.e. to 2000 gm.
Ditto.
Ditto.
Say reaction + 4.2%.
Ditto.
Say 900 gm.
Ditto.
Add peptone, 2%, 18 gm.
Ditto.
Prob.+4'S to +4.7.
Ditto.
To the goo gm. of meat infusion (containing now peptone and salt) add 900 gm. of the 3% agar jelly described at the head of this column.
10. Heat over boiling water (or steam) bath thirty minutes.
11. Restore weight lost by evaporation to original weight of filtered meat infusion, i.e. that on which the percentage of peptone and salt, etc, were cal- culated, — 1800 gm.


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