Essentials of Bacteriology Being a Concise And Systematic Introduction to the

M V Michael Valentine Ball

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59.
Bacillus of Pneumonia in Saliva. (After Biondl. ) Properties. They are without self-movement ; do not liquefy gelatine.
Growth. Grow only at high temperature, 35 C. ; are facul- tative anserobic. The culture media must be slightly alkaline ; the growth is slow.
Colonies on Gelatine Plates. Since the temperature must be somewhat elevated, the gelatine media need to be thicker than usual (15 per cent, gelatine), in order to keep it solid, and a temperature of 24 C. Used. Little round white co...lonies, some- what granular in the centre, growing very slowly. 8 114 ESSENTIALS OF BACTERIOLOGY.
Stab Cultures. Along the needle-track small separate white granules, one above the other, like a string of beads.
Stroke Culture. On agar, transparent, almost invisible little drops resembling dew moisture.
Bouillon. They grow better here than in the other media, remaining alive a longer period of time.
Staining. Takes Gram's method and the other aniline stains very readily. The capsule stained the same way as that of the Friedlande-r bacillus.

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