Exercises in Bacteriology And Diagnosis for Veterinary Students And Practitioners

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Particu- lar attention should be given to its morphology and staining properties.
113. Work for this exercise. Examine microscopically, in hanging-drop and stained cover-glass preparations, the bacteria from the glycerin agar and Loeffler's blood-serum cultures.
Stain withi alkaline methylene blue and note especially the way' the bacteria stain. The preparation should be stained for fully 5 minutes and then decolorized for a few seconds in 95% alcohol.
Stain a few preparations after Neisser's m
...ethod (the staining solutions will be furnished) and compare with the methylene- blue stain.
"jd LABORATORY BACTERIOLOGY Note with special care the morphology of the bacteria and make a drawing of a few of them.
Examine very caxefully a guinea pig (furnished) which has died from the effect of inoculation with diphtheria organisms.
114. Neisser's method of staining diphtheria bacteria. Neisser has recommended the foUbwirig method of staining, in which 2 solutions are employed ; viz., 1. One gram of methylene blue (Griibler's) is dissolved in 20 cc.


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