Exercises in Practical Physiological Chemistry

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of the salted plasma add an equal volume of a saturated solution of sodium chloride. A precipitate of fibrinogen is formed. Allow the tube to stand for a few miuutes CH. VI.J THE COAGULATION OF BLOOD. 73 and then filter through a small paper. Scrape the precipitate off the paper and treat it with about 5 c.c.
of 5 per cent. NaCl. The fibrinogen dissolves. Divide the solution into two portions, C and D. To C add two drops of fibrin ferment. Place both tubes in the warm bath and observe them at i
...ntervals. C clots rapidly; D very slowly.
161. The heat-coagulation of fibrinos:en. Heat 5 c.c. of salted plasma as in Ex. 9. Notice the coagu- lation of fibrinogen which occurs at 56°C. Continue heating to 6o°C. and then filter. Dilute the filtrate as in Ex. 159 ; add fibrin ferment, and place on the warm bath. Coagulation does not occur.
Note. — Fibrinogen is a globulin, but differs from other proteins of this class in being precipitated by half-saturation with NaCl.
The precipitate obtained by saturating plasma with NaCl consists of a mixture of fibrinogen and serum-globulin which is known cis plasmine.


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