Handbook of Practical Botany for the Botanical Laboratory And Private Student

Cover Handbook of Practical Botany for the Botanical Laboratory And Private Student
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— [Ed.] MICROTOMY : EMBEDDING 449 suitably arranged in this new quantity of paraffin. We then cut the paraffin into small cubes or rectangular prisms. The paraffin cubes or prisms prepared in this way are fused to a large block of paraffin by means of a hot needle. This large block will be of such size as lends itself to ready fixing in the object holder of the micro- tome, and is high enough to enable suitable sections to be cut.
The microtome razor must be fairly parallel to a side of the ang
...ular paraffin block in which the object is contained. If, how- ever, as with such embedded preparations only quite exceptionally happens, we cut, not with a cross but an obliquely-fixed razor, then the paraffin containing the preparation should be cut in the form of a three-sided prism, and care taken that the side next the razor should be arranged parallel to it.
The transfer from alcohol to paraffin through the intermediary of chloroform has been proved best for our purposes ; but on the other hand cedar oil has been specially recommended, and has the advantage of great simplicity.


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