Histological Technique
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Formulas (a) and (b) may be used with sections and in the same way as eosin.
§ 107. Picric Acid. A counter-stain useful after carmine or hema- toxylin.
Employ a 1-5% alcoholic solution, or simply add to the alcohol used in dehydration. It washes out the hematoxylin and is useful as a differ- entiator of this stain, with which it is well to overstain somewhat if it is desired to counter-stain with picric acid.
§ 108. Orange G. An excellent acid stain that may be employed as counter-stain and dif...ferentiator after hematoxylin, gentian violet, etc.
Employ a 2 to 4% aqueous solution which may with advantage be slightly acid (Hcl.) ; in the last event, sections should be rinsed well be- fore the mounting. It may with good effect be combined with eosin, eryth- rosin or fuchsin acid.
§ 109. Fuchsin Acid (Rubin S., Magenta S.). Like Congo red, an indi- cator, red in acid of neutral solutions, bleached by alkali. It is a valuable plasma stain, but requires care in its use because of its sensitiveness to alkali.
What to read after Histological Technique?
You can find similar books in the "Read Also" column, or choose other free books by Kingsbury, B. F. (Benjamin Freeman), 1872-1946 to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Formulas (a) and (b) may be used with sections and in the same way as eosin.
§ 107. Picric Acid. A counter-stain useful after carmine or hema- toxylin.
Employ a 1-5% alcoholic solution, or simply add to the alcohol used in dehydration. It washes out the hematoxylin and is useful as a differ- entiator of this stain, with which it is well to overstain somewhat if it is desired to counter-stain with picric acid.
§ 108. Orange G. An excellent acid stain that may be employed as counter-stain and dif...ferentiator after hematoxylin, gentian violet, etc.
Employ a 2 to 4% aqueous solution which may with advantage be slightly acid (Hcl.) ; in the last event, sections should be rinsed well be- fore the mounting. It may with good effect be combined with eosin, eryth- rosin or fuchsin acid.
§ 109. Fuchsin Acid (Rubin S., Magenta S.). Like Congo red, an indi- cator, red in acid of neutral solutions, bleached by alkali. It is a valuable plasma stain, but requires care in its use because of its sensitiveness to alkali.
What to read after Histological Technique?
You can find similar books in the "Read Also" column, or choose other free books by Kingsbury, B. F. (Benjamin Freeman), 1872-1946 to read onlineMoreLess
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