Immunochemistry; the Application of the Principles of Physical Chemistry to the Study of the Biological Antibodies

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^ a 300 ■^'" 150 ^' 100 ^ This is evidently true only if the hsemolytic action is of such a magnitude as it is possible to reach with the quan- tity of b present ; and this circumstance indicates that the said rule is only a rather rough approximation. The rule stated leads to the consequence that the maximum appears at a lower value of a for a lower value of b, and the values of a at the maximum are nearly proportional to the value of (5 (80 : 10 = 8 ; 50 : 6 = 8.3 ; 30 : 4 = 7.5).
It had been
... observed in different investigations that the quantity of alexin necessary to produce complete haemolysis is the less the greater the quantity of immune- body that had been used for the " sensibilisation " of the erythrocytes.^ This matter was subjected to a closer investigation by Morgenroth and Sachs.^ They found that in one case (viz. haemolysis of sheep erythrocytes by means of immune-bodies from goat blood and alexin from guinea-pig serum) the quantity (3) of alexin was often nearly inversely proportional to the quantity («) of immune-body used.

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